Occurrence of IGG antibodies anti-TOXOPLASMA GONDII and NEOSPORA CANINUM in cattle raised in family agricultural properties in Realeza, Parana, Brazil

This cross-sectional study evaluates the presence of antibodies against Neospora caninum and Toxoplasma gondii in cattle raised in Realeza, PR. There was a seroprevalence of N. caninum and T. gondii of 87.5% and 67.9%, respectively in the properties assessed. The frequencies of T. gondii and N. caninum in the animals were 41.1% and 55.1%, respectively. The studied coccidia are widely distributed in dairy cows reared on family farms in the municipality of Realeza, PR. Sanitary control for reproductive diseases must be adopted to prevent miscarriages and the economic damage caused by the disease. Future studies should be performed to investigate how widespread these pathogens are in cattle herds in southwestern Parana.

Uso de complexo homeopático reduz o parasitismo por carrapatos e amplia o intervalo de tempo entre tratamentos com acaricidas / Homeopathic complex decreases tick parasitism and increases the interval between treatments with acaricides

O presente trabalho avaliou a segurança e eficácia de 2 formulações homeopáticas no tratamento populacional de bovinos naturalmente parasitados com carrapatos da espécie Rhipicephalus (Boophilus) microplus. Foram realizados 2 experimentos utilizando complexos homeopáticos distintos, ambos com 20 vacas adultas, raça holandesa, em diferentes estágios de lactação, distribuídas em grupo controle (n= 10) e grupo tratado (n= 10) num estudo randomizado com duplo cego. A contagem de carrapatos foi registrada nos dias 7, 14, 21 e 28 após o tratamento homeopático e, posteriormente, em intervalos de 15 dias até o dia 140 do Experimento 01 e o dia 197 do Experimento 02. A formulação homeopática utilizada no Experimento 01 apresentou eficácia de 21,1%, sendo necessária intervenção com tratamento convencional durante o período de 140 dias. A eficácia do complexo homeopático no Experimento 02 foi de 6,7%. Os resultados foram seguros e eficazes na terapia antiparasitária, tendo bons resultados quando complementados com o uso racional e correto de acaricidas. (AU)

In the present study evaluated the efficacy of 2 homeopathic formulas in the treatment of cattle naturally with Rhipicephalus (Boophilus) microplus ticks. Two experiments were carried out using different combinations of homeopathic medicines, with 20 adult Holstein cows, in different stages of lactation, allocated to the control (n= 10) and treated (n= 10) groups in a randomized double-blind placebo-controlled trial. Tick count was performed on days 7, 14, 21 and 28 after homeopathic treatment ,and then at 15-day intervals up to day 140 in Experiment 1 and day 197 in Experiment 2. Efficacy in Experiment 1 was 21.1%, while conventional intervention was required along the 140-day period. Efficacy in Experiment 2 was 6.7%. The results indicate that the tested homeopathic formulas were safe and efficacious, outcomes being satisfactory when homeopathic treatment was combined with rational and correct conventional treatment. (AU)

Enzootic instability for bovine anaplasmosis on family farms located in southwestern Paraná, Brazil / Instabilidade enzoótica para anaplasmose bovina em propriedades de agricultura familiar do sudoeste paranaense, Brasil

Abstract The objective of this study was to assess the occurence of animals seropositive for Anaplasma marginale in the municipality of Realeza, Paraná State, Brazil. Blood samples were collected from 344 cows on 18 small farms in the municipality of Realeza-PR. The animals’serum samples were forwarded to the Federal University of Fronteira do Sul, in order to investigate the occurrence of anti-A. marginale IgG antibodies by an enzyme-linked immunosorbent assay commercial kit. IgG antibodies to A. marginale were detected in cattle from 77.7% of the farms. To the best author's knowledge, this is the first report of occurrence of A. marginale in cattle in southwestern Paraná. The serological assay showed that 24.4% of the animals were seropositive, thus characterizing the location investigated as an area of enzootic instability for the disease. The family farms located in the municipality of Realeza-PR showed enzootic instability for bovine anaplasmosis. It is necessary to conduct disease monitoring programs in association with preventive measures in order to ensure the sanitary quality of the herds and to reduce economic losses for the farmers. In addition, it is essential to implement educational extension actions that allow farmers to acquire knowledge, attitudes and perceptions regarding the risk factors that contribute towards herd A. marginale-infection.

Resumo O objetivo deste estudo foi avaliar a ocorrência de animais soropositivos para Anaplasma marginale, no município de Realeza, Estado do Paraná, Brasil. Foram colhidas amostras de sangue de 344 fêmeas bovinas provenientes de 18 propriedades rurais do município de Realeza - PR. Amostras de soro dos animais foram encaminhadas à Universidade Federal da Fronteira Sul, para realização do Ensaio Imunoenzimático Indireto para pesquisa de anticorpos IgG anti- Anaplasma marginale por meio de kit comercial. Anticorpos IG anti-A. marginale foram detectados em 77,7% das propriedades. Trata-se do primeiro registro da ocorrência de A. marginale no Sudoeste paranaense. A sorologia evidenciou 24,4% de animais soropositivos, caracterizando o local pesquisado como área de instabilidade enzoótica para a doença. As propriedades de agricultura familiar, localizadas no município de Realeza-PR, apresentaram instabilidade enzoótica para anaplasmose bovina. É necessário que programas de monitoramento da enfermidade sejam realizados em conjunto com medidas de prevenção, visando garantir qualidade sanitária do rebanho e reduzir perdas econômicas dos produtores rurais. Além disso, é fundamental a realização de ações de extensão que viabilizem a aquisição de conhecimento, atitude e percepção dos criadores diante dos fatores de risco contribuintes para a infecção por A. marginale do rebanho.

Enzootic instability for bovine anaplasmosis on family farms located in southwestern Paraná, Brazil.

The objective of this study was to assess the occurence of animals seropositive for Anaplasma marginale in the municipality of Realeza, Paraná State, Brazil. Blood samples were collected from 344 cows on 18 small farms in the municipality of Realeza-PR. The animals'serum samples were forwarded to the Federal University of Fronteira do Sul, in order to investigate the occurrence of anti-A. marginale IgG antibodies by an enzyme-linked immunosorbent assay commercial kit. IgG antibodies to A. marginale were detected in cattle from 77.7% of the farms. To the best author's knowledge, this is the first report of occurrence of A. marginale in cattle in southwestern Paraná. The serological assay showed that 24.4% of the animals were seropositive, thus characterizing the location investigated as an area of enzootic instability for the disease. The family farms located in the municipality of Realeza-PR showed enzootic instability for bovine anaplasmosis. It is necessary to conduct disease monitoring programs in association with preventive measures in order to ensure the sanitary quality of the herds and to reduce economic losses for the farmers. In addition, it is essential to implement educational extension actions that allow farmers to acquire knowledge, attitudes and perceptions regarding the risk factors that contribute towards herd A. marginale-infection.

Alterações hematológicas e bioquímicas causadas por Anaplasma marginale em bovinos com aptidão leiteira da região Sudoeste do Paraná / Changes hematological and biochemical caused by Anaplasma marginale in dairy cattle suitability of southwest region Paraná

O objetivo deste trabalho foi descrever as complicações hematológicas e bioquímicas em bovinos da região Sudoeste do Paraná infectados por Anaplasma marginale, comparando estas alterações com os parâmetros apresentados por bovinos sadios da mesma região. Foram avaliadas 40 vacas com aptidão leiteira, sendo 20 clinicamente suspeitas de estarem infectadas pelo parasito e outras 20 sadias, que serviram como grupo controle. O hemograma foi realizado em contador hematológico automático. Para a contagem diferencial de células brancas e pesquisa do agente realizou-se esfregaço sanguíneo. As análises de albumina, proteínas totais (PT), uréria, creatinina, aspartato-aminotrasferase, e gama-glutamiltransferase séricas, foram realizadas em analisador bioquímico semi-automático. Os resultados obtidos foram avaliados através do programa SPSS, versão 20.0, sendo submetidos ao Teste t de Student. O diagnóstico foi realizado pela identificação do hemoparasito em esfregaço sanguíneo. Os animais infectados apresentaram uma parasitemia que variou de 11 a 20%. Constatou-se diferença estatística significante (p<0,01), entre os seguintes parâmetros: hematócrito das infectadas (17,99±1,49%) e sadias (29,38±0,96); número de hemácias das infectadas (3,71±0,39x106 /µL) e sadias (7,23±0,37x106 /µL); hemoglobina das infectadas (6,08±0,65g/dL) e sadias (9,96±0,33g/dL); VCM das infectadas (52,60±3,07fL) e sadias (41,34±1,31fL); monócitos das infectadas (1353,27±373,61/µL) e sadias (517,84±113,22/µL); PT (proteínas totais) das infectadas (7,60±0,18g/dL) e sadias (5,90±0,28g/dL). Os animais infectados apresentaram acentuada anemia e aumento sérico das PT. Conclui-se que estes hemoparasitos provocam monocitose e sinais clínicos graves decorrentes da anemia acentuada, porém não alteram os marcadores da função hepática e renal de bovinos na forma clínica de Anaplasmose.

The objective of this study was to describe the hematological and biochemical complications in cattle of southwestern Paraná region infected with Anaplasma, comparing these changes with the parameters presented by healthy cattle from the same region. We evaluated 40 cows with milk aptitude, 20 clinically suspected of being infected by the parasite and other 20 healthy, which served as a control group. Blood counts were performed in automated hematology counter. For the differential white cell count and agent search was held smears. Albumin analysis, total protein (TP), urea, creatinine, aspartate-aminotransferase and serum gamma-glutamyl transferase were carried out in semi-automatic biochemical analyzer. The results were evaluated using SPSS, version 20.0, and submitted to the Student t test. The diagnosis was made by identifying the hemoparasite in blood smears. Infected mice had a parasitemia ranging from 11 to 20%. We found statistically significant differences (p<0.01) among the following: hematocrit of infected (17,99±1,49%) and healthy controls (29,38±0,96%); number of red blood cells infected (3,71±0,39x106 / µL) and healthy controls (7,23±0,37x106 /µL); of infected hemoglobin (6,08±0,65g/dL) and healthy controls (9,96±0,33g/dL); VCM infected (52,60±3,07fL) and healthy controls (41,34±1,31fL); of infected monocytes (1353,27±373,61/µL) and healthy controls (517,84±113,22/µL); PT (total protein) of infected (7,60±0,18g/dL) and healthy controls (5,90±0,28g/dL). Infected animals showed marked anemia and serum increase in PT. We conclude that these blood parasites cause monocytosis and severe clinical signs resulting from severe anemia, but do not alter markers of liver and renal function of cattle in the clinical form of Anaplasmosis.

Metabolic alterations in broiler chickens experimentally infected with sporulated oocysts of Eimeria maxima.

Metabolic and morphometric alterations of the duodenal villi caused by parasitism of chickens by Eimeria maxima were evaluated, using 100 male Cobb birds, randomly distributed into two groups (control and infected). The infected group was inoculated with 0.5 ml of a solution containing 5 × 10³ sporulated oocysts of Eimeria maxima. Ten birds per sample were sacrificed on the 6th, 11th, 22nd and 41st days post-infection (dpi). In order to evaluate the alterations, samples of duodenum, jejunum and ileum fragments were collected after necropsy for histological analysis. Villus biometry was determined by means of a slide graduated in microns that was attached to a binocular microscope. To evaluate the biochemical data, 5 ml of blood were sampled from the birds before sacrifice. The statistical analyses were performed using the GraphPad 5 statistical software for Windows. Tukey's multiple comparison test (p <0.05) was performed for the different dpi's and the unpaired t test for the difference between the groups. Infection by E. maxima causes both qualitative and quantitative alterations to the structure of the intestinal villi, thereby interfering with the absorption of nutrients such as calcium, phosphorus, magnesium, protein and lipids, with consequent reductions in the birds' weights.

Metabolic alterations in broiler chickens experimentally infected with sporulated oocysts of Eimeria maxima / Alterações metabólicas em frangos de corte infectados experimentalmente com oocistos esporulados de Eimeria maxima

Metabolic and morphometric alterations of the duodenal villi caused by parasitism of chickens by Eimeria maxima were evaluated, using 100 male Cobb birds, randomly distributed into two groups (control and infected). The infected group was inoculated with 0.5 ml of a solution containing 5×103 sporulated oocysts of Eimeria maxima. Ten birds per sample were sacrificed on the 6th, 11th, 22nd and 41st days post-infection (dpi). In order to evaluate the alterations, samples of duodenum, jejunum and ileum fragments were collected after necropsy for histological analysis. Villus biometry was determined by means of a slide graduated in microns that was attached to a binocular microscope. To evaluate the biochemical data, 5 ml of blood were sampled from the birds before sacrifice. The statistical analyses were performed using the GraphPad 5 statistical software for Windows. Tukey's multiple comparison test (p <0.05) was performed for the different dpi's and the unpaired t test for the difference between the groups. Infection by E. maxima causes both qualitative and quantitative alterations to the structure of the intestinal villi, thereby interfering with the absorption of nutrients such as calcium, phosphorus, magnesium, protein and lipids, with consequent reductions in the birds' weights.

Foram avaliadas alterações metabólicas e morfométricas das vilosidades intestinais causadas pelo parasitismo de frangos por Eimeria maxima, sendo utilizadas 100 aves da linhagem Coob, machos, distribuídos aleatoriamente em dois grupos experimentais: grupo controle, inoculado com 0,5 ml de água destilada; grupo infectado, inoculado com 0,5ml de solução contendo 5×103 oocistos esporulados de Eimeria maxima. Foram sacrificadas 10 aves por coleta no 0, 6, 11, 22 e 41 dias pós-infecção. Para avaliar as alterações foram retiradas, após necropsia, amostras de fragmentos do duodeno, jejuno e íleo para análise histológica. A determinação da biometria de vilosidades foi realizada por meio de lâmina milimetrada acoplada a um microscópio binocular. Para avaliação dos dados bioquímicos foram coletados 5 ml de sangue das aves antes da eutanásia. As análises estatísticas foram realizadas, utilizando-se o programa estatístico Graphpad Prism. 5 – Windows e realizado o teste de comparações múltiplas de Tukey (p <0,05) para os diferentes dpi's e o Teste T não Pareado para diferença entre os grupos. A infecção por E. maxima provoca alterações qualitativas e quantitativas das vilosidades intestinais, interferindo na absorção de nutrientes, como cálcio, fósforo, magnésio, proteínas e lipídios, com consequente redução no peso das aves.

Parasitoses intestinais em crianças residentes na comunidade Ribeirinha São Francisco do Laranjal, município de Coari, Estado do Amazonas, Brasil / Intestinal parasitoses in children living in the riverbank community of São Francisco do Laranjal, municipality of Coari, state of Amazonas, Brazil

Foram examinadas 65 crianças na faixa etária compreendida entre 1 e 12 anos, sendo o exame coproparasitológico realizado de acordo com a técnica de sedimentação espontânea. Os examesevidenciaram 83,1por cento de positividade enteroparasitária, dos quais 57,4por cento se referiam a crianças dosexo masculino e 42,6por cento, do sexo feminino. As espécies de parasitos encontrados foram: Ascaris lumbricoides (53,7por cento), Trichuris trichiura (16,6por cento), ancilostomatídeos (15por cento), Entamoeba coli(9,2por cento) e Giardia duodenalis (5,5por cento). Os resultados positivos revelaram 55,5por cento de monoparasitismo por A. lumbricoides, 37,1por cento de biparasitismo e 7,4por cento de poliparasitismo. Quanto à associação parasitária, 68,5por cento das infecções foram ocasionadas por helmintos, 7,4por cento somente por protozoários e 24,1por cento tanto por helmintos quanto por protozoários.

Coccidiosis in broiler chickens raised in the Araguaína region, State of Tocantins, Brazil.

Eimeriosis is responsible for causing serious problems in poultry, mainly characterized by reduced weight gain and abnormalities of food conversion efficiency, thereby causing great economic losses. The aim of this study was to evaluate the epidemiology of eimeriosis in broiler chickens in the Araguaina region, State of Tocantins, Brazil. Samples from five farm properties were collected and sent to the Hygiene and Public Health Laboratory, School of Veterinary Medicine and Zootechnics, Federal University of Tocantins. From the parasitological analysis, it was shown that all the properties examined were positive for Eimeria species. 63.1% of the sheds were positive, with findings of oocysts of E. maxima, E. acervulina, E. mitis and E. tenella. It was concluded that all properties evaluated were positive for four species of the genus Eimeria, thus demonstrating that the sanitary strategies followed in poultry rearing had flaws that allowed pathogens to spread in poultry pens.

Coccidiosis in broiler chickens raised in the Araguaína region, State of Tocantins, Brazil / Coccidiose em frangos de corte criados na região de Araguaína, estado do Tocantis, Brasil

Eimeriosis is responsible for causing serious problems in poultry, mainly characterized by reduced weight gain and abnormalities of food conversion efficiency, thereby causing great economic losses. The aim of this study was to evaluate the epidemiology of eimeriosis in broiler chickens in the Araguaina region, State of Tocantins, Brazil. Samples from five farm properties were collected and sent to the Hygiene and Public Health Laboratory, School of Veterinary Medicine and Zootechnics, Federal University of Tocantins. From the parasitological analysis, it was shown that all the properties examined were positive for Eimeria species. 63.1 percent of the sheds were positive, with findings of oocysts of E. maxima, E. acervulina, E. mitis and E. tenella. It was concluded that all properties evaluated were positive for four species of the genus Eimeria, thus demonstrating that the sanitary strategies followed in poultry rearing had flaws that allowed pathogens to spread in poultry pens.

A eimeriose é responsável por causar graves problemas avícolas caracterizados, principalmente, por redução do ganho de peso e alteração na conversão alimentar, gerando grandes perdas econômicas. O objetivo deste trabalho foi avaliar a epidemiologia da eimeriose em frangos de corte na região de Araguaína, Estado do Tocantins, Brasil. Amostras de cinco propriedades foram colhidas e encaminhadas ao Laboratório de Higiene e Saúde Pública da Escola de Medicina Veterinária e Zootecnia da Universidade Federal do Tocantins. Após análise parasitológica, comprovou-se que todas as propriedades avaliadas apresentaram positividade para espécies do gênero Eimeria, tendo 63,1 por cento dos galpões positivos, sendo encontrados oocistos de E. maxima, E. acervulina, E. mitis e E. tenella. Conclui-se que todas as propriedades avaliadas, apresentaram positividade para quatro espécies do gênero Eimeria comprovando que as estratégias sanitárias adotadas nas criações apresentam falhas que possibilitam a disseminação do patógeno nos lotes avícolas.

Ocorrência de anticorpos anti-Neospora caninum em vacas lactantes do município de Araguaína, estado do Tocantins, Brasil / Occurrence of antibodies anti Neospora caninum in lactating cows from the municipality of Araguaina, Tocantins State, Brazil

Este estudo teve como objetivo determinar a presença de anticorpos anti-Neospora caninum em vacas lactantes pertencentes às propriedades rurais do município de Araguaína, Tocantins, Brasil. Para isso, amostras de sangue de 192 vacas foram selecionadas aleatoriamente e analisadas pela Reação de Imunofluorescência Indireta (RIFI) para detecção de anticorpos da classe IgGanti-Neospora caninum. Foram utilizados como antígeno taquizoítos do isolado Nc-1 do parasito, cultivados em células Vero, sendo o ponto de corte correspondente aos títulos superiores a 1:200. Realizou- se ainda a titulação das amostras positivas até a diluição 1:6.400. Das amostras analisadas,48 (25por cento) mostraram-se positivas e, nas propriedades analisadas, a prevalência foi de 12,5por cento a 33,33por cento, o que revelou exposição dos animais ao parasito no município de Araguaína, sendo este oprimeiro registro no estado do Tocantins.

The aim of this study was to investigate the occurrence of anti-Neospora caninum antibodies in lactating cows from farms in the municipality of Araguaína, Tocantins State, Brazil. Blood samples from 192 randomly selected cows were collected and analyzed by Indirect Immunofluorescence (IIF) in order to detect IgG anti-Neospora caninum antibodies. Tachyzoites of the Nc-1 isolate of N. caninum, cultivated in Vero cells, were used as antigen and the cut-off point was titers above 1:200. Titration of positive samples until the dilution 1:6,400 was also performed. Forty-eight samples (25%) were serum positive; the prevalence in the farms was between 12.5% and 33.3% revealing the animal’s exposition to the parasite in the municipality of Araguaína. This is the first record of this parasite in the State of Tocantins.

Systemic inflammatory response indicators in rabbits (Oryctolagus cuniculus) experimentally infected with sporulated oocysts of Eimeria stiedai (Apicomplexa: Eimeriidae).

Hemograms and acute-phase proteins in adult male New Zealand White rabbits that had been experimentally infected orally with sporulated oocysts of Eimeria stiedai were evaluated over a 28-day period. Fifty animals were used, divided into two groups: group A infected with 1 × 10(4) sporulated oocysts of E. stiedai and group B inoculated with distilled water. On the seventh day after infection, the infected animals presented anemia and leukocytosis with neutrophilia and monocytosis. Protein fractionation by means of electrophoresis identified 19 acute-phase proteins with molecular weights ranging from 24 to 238 kD. Ceruloplasmin, transferrin and haptoglobin showed high levels on the seventh day after infection, with gradual increases in their concentrations until the end of the experimental period. Thus, from the data of the present study, E. stiedai is considered to be a pyogenic etiological agent for which the infection level can be monitored through the leukocyte count and serum concentrations of ceruloplasmin, transferrin and haptoglobin, and these can be recommended as complementary tests.

Systemic inflammatory response indicators in rabbits (Oryctolagus cuniculus) experimentally infected with sporulated oocysts of Eimeria stiedai (Apicomplexa: Eimeriidae) / Indicadores da resposta inflamatória sistêmica em coelhos (Oryctolagus cuniculus) infectados experimentalmente com oocistos esporulados de Eimeria stiedai (Apicomplexa: Eimeriidae)

Hemograms and acute-phase proteins in adult male New Zealand White rabbits that had been experimentally infected orally with sporulated oocysts of Eimeria stiedai were evaluated over a 28-day period. Fifty animals were used, divided into two groups: group A infected with 1 × 10(4) sporulated oocysts of E. stiedai and group B inoculated with distilled water. On the seventh day after infection, the infected animals presented anemia and leukocytosis with neutrophilia and monocytosis. Protein fractionation by means of electrophoresis identified 19 acute-phase proteins with molecular weights ranging from 24 to 238 kD. Ceruloplasmin, transferrin and haptoglobin showed high levels on the seventh day after infection, with gradual increases in their concentrations until the end of the experimental period. Thus, from the data of the present study, E. stiedai is considered to be a pyogenic etiological agent for which the infection level can be monitored through the leukocyte count and serum concentrations of ceruloplasmin, transferrin and haptoglobin, and these can be recommended as complementary tests.

O hemograma e proteínas de fase aguda foram avaliados durante 28 dias em coelhos adultos, machos, raça branco Nova Zelândia, infectados experimentalmente, via oral, com oocistos esporulados de Eimeria stiedai. Foram usados 50 animais distribuídos em dois grupos: grupo A infectado com 1 × 10(4) oocistos esporulados de E. stiedai e grupo B inoculado com água destilada. No 7º dia após a infecção (dpi), os animais infectados tiveram anemia, leucocitose com neutrofilia e monocitose. O método de fracionamento de proteínas por eletroforese identificou 19 proteínas de fase aguda com pesos moleculares que variaram entre 24 e 238 kD. A ceruloplasmina, transferrina e haptoglobina tiveram níveis elevados no 7° dpi com aumento progressivo de suas concentrações até o término do período experimental. Desta forma, considerando-se os dados encontrados no presente estudo, E. stiedai é considerado um agente etiológico piogênico que pode ter sua infecção monitorada por determinação do leucograma e das concentrações séricas de ceruloplasmina, transferrina e haptoglobina, podendo ser estes recomendados como exames complementares.

Detection of antibodies against Babesia bovis and Babesia bigemina in calves from the region of Araguaína, State of Tocantins, Brazil.

The aim of this study was to determine the seroprevalence of antibodies against B. bovis and B. bigemina in calves from the region of Araguaína, State of Tocantins, Brazil. In this research we used sera obtained from 506 calves, from both genders and of 8 to 24 months old, to detect antibodies by indirect Enzyme-Linked Immunosorbent Assay (ELISA-test). Statistical analysis of the data was performed using the Chi-square (χ²) test with Yates correction. The seroprevalence obtained was 90.5 and 91.7% for B. bigemina and B. bovis, respectively, characterizing the region as an area of enzootic stability for the species analyzed. The seroprevalence to B. bovis showed higher positivity among calves 19-24 months old.

Detection of antibodies against Babesia bovis and Babesia bigemina in calves from the region of Araguaína, State of Tocantins, Brazil / Detecção de anticorpos anti-Babesia bovis e anti-Babesia bigemina em bezerros na região de Araguaína, Estado do Tocantins, Brasil

The aim of this study was to determine the seroprevalence of antibodies against B. bovis and B. bigemina in calves from the region of Araguaína, State of Tocantins, Brazil. In this research we used sera obtained from 506 calves, from both genders and of 8 to 24 months old, to detect antibodies by indirect Enzyme-Linked Immunosorbent Assay (ELISA-test). Statistical analysis of the data was performed using the Chi-square (χ2) test with Yates correction. The seroprevalence obtained was 90.5 and 91.7 percent for B. bigemina and B. bovis, respectively, characterizing the region as an area of enzootic stability for the species analyzed. The seroprevalence to B. bovis showed higher positivity among calves 19-24 months old.

O objetivo desse estudo foi determinar a soroprevalência de anticorpos anti-B. bovis e anti-B. bigemina em bezerros da região de Araguaína, Estado do Tocantins, Brasil. Nesta pesquisa foram coletadas 506 amostras de soros de bezerros com faixa etária entre 8 e 24 meses, fêmeas e machos, as quais foram processadas pelo ensaio imunoenzimático indireto (ELISA-teste). Como análise estatística utilizou-se o Qui-Quadrado (χ2) com correção de Yates. As prevalências obtidas em bezerros foram de 90,5 e 91,7 por cento para B. bigemina e B. bovis, respectivamente, caracterizando esta região como de estabilidade enzoótica para as espécies analisadas. A soroprevalência para B. bovis apresentou maior positividade entre os bezerros com faixa etária de 19-24 meses.